This study presents paleoenvironmental data based on pollen, elemental and isotopic compositions of organic matter (TOC, N, δ(13)C and δ(15)N) and (14)C dating of 170 cm lake sediment record. Samplings have been made in Lagoa Grande at Parque Estadual Turístico do Alto Ribeira - PETAR, Southern São Paulo State, Southeastern Brazil. The variations in relative frequencies (in percentage) of arboreal pollen along the core range between 40 and 80%. The δ(13)C values ranged from -23% to -30% and C/N of ~10 to 15, indicating the contribution of terrestrial C(3) plants and algae in the sediment organic matter. The δ(15)N results presented values from 3 to 4.5%, also suggesting a mixture of algae and terrestrial C(3) plants. The (14)C dating indicates modern age for the shallow horizons to ~1030 BP at the base of the core. A probable wetter climate in the period of ~370 BP to ~340 BP was inferred from the data set, which corresponds to a part of the period covered by the Little Ice Age (LIA).