Isolation of Mycobacterium tuberculosis strains with a silent mutation in rpoB leading to potential misassignment of resistance category

J Clin Microbiol. 2011 Jul;49(7):2688-90. doi: 10.1128/JCM.00659-11. Epub 2011 May 11.

Abstract

Our study provides an alert regarding the transmission of rifampin-susceptible strains of Mycobacterium tuberculosis with a silent substitution in codon 514 of rpoB. Among 1,450 cases, we identified 12 isolates sharing this mutation and related restriction fragment length polymorphism (RFLP) types. The mutation impaired hybridization with the wild-type probes in three independent commercial assays, which could lead to misassignment of resistance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution / genetics
  • Antitubercular Agents / pharmacology*
  • Bacterial Proteins / genetics*
  • DNA, Bacterial / genetics
  • DNA-Directed RNA Polymerases
  • Diagnostic Errors*
  • Drug Resistance, Bacterial*
  • Genotype
  • Humans
  • Microbial Sensitivity Tests / methods
  • Molecular Typing
  • Mutation, Missense*
  • Mycobacterium tuberculosis / classification
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / isolation & purification
  • Nucleic Acid Hybridization / methods
  • Polymorphism, Restriction Fragment Length
  • Rifampin / pharmacology*

Substances

  • Antitubercular Agents
  • Bacterial Proteins
  • DNA, Bacterial
  • rpoB protein, Mycobacterium tuberculosis
  • DNA-Directed RNA Polymerases
  • Rifampin