Efficient implementation of RNA interference in the pathogenic yeast Cryptococcus neoformans

J Microbiol Methods. 2011 Aug;86(2):156-9. doi: 10.1016/j.mimet.2011.04.014. Epub 2011 Apr 29.

Abstract

An improved method has been developed for RNA interference in Cryptococcus neoformans, using opposing promoters to facilitate cloning and RNA interference targeting URA5 to allow selection of cells in which silencing is most effective. These advances significantly reduce the variability of silencing and the effort required for interference plasmid construction.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cryptococcus neoformans / genetics*
  • Cryptococcus neoformans / growth & development*
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • Genetics, Microbial / methods*
  • Humans
  • Molecular Sequence Data
  • Plasmids*
  • RNA Interference*
  • Sequence Analysis, DNA

Substances

  • DNA, Fungal

Associated data

  • GENBANK/HQ455038