Evaluation of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry for identification of Candida parapsilosis, C. orthopsilosis and C. metapsilosis

I Quiles-Melero; J García-Rodríguez; A Gómez-López; J Mingorance

doi:10.1007/s10096-011-1277-z

Evaluation of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry for identification of Candida parapsilosis, C. orthopsilosis and C. metapsilosis

Eur J Clin Microbiol Infect Dis. 2012 Jan;31(1):67-71. doi: 10.1007/s10096-011-1277-z. Epub 2011 May 6.

Authors

I Quiles-Melero¹, J García-Rodríguez, A Gómez-López, J Mingorance

Affiliation

¹ Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de La Castellana 261, Madrid, Spain.

PMID: 21547602
DOI: 10.1007/s10096-011-1277-z

Abstract

We have evaluated matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry for the rapid identification of Candida parapsilosis, C. orthopsilosis and C. metapsilosis. A total of 103 isolates, including reference strains and clinical isolates, were identified by pyrosequencing of the ITS1 region and then assay by MALDI-TOF mass spectrometry. Concordance between the two methods was 100%, showing that MALDI-TOF may be useful as a rapid and reliable method for discrimination of species within the C. parapsilosis group.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Candida / classification*
Candida / genetics
Candida / isolation & purification*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics*
DNA, Ribosomal Spacer / chemistry
DNA, Ribosomal Spacer / genetics
Genes, rRNA
Sequence Alignment
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

DNA, Bacterial
DNA, Ribosomal Spacer