Single-shot ultrabroadband two-dimensional electronic spectroscopy of the light-harvesting complex LH2

Opt Lett. 2011 May 1;36(9):1665-7. doi: 10.1364/OL.36.001665.

Abstract

Here we present two-dimensional (2D) electronic spectra of the light-harvesting complex LH2 from purple bacteria using coherent pulses with bandwidth of over 100 nm FWHM. This broadband excitation and detection has allowed the simultaneous capture of both the B800 and B850 bands using a single light source. We demonstrate that one laser pulse is sufficient to capture the entire 2D electronic spectrum with a high signal-to-noise ratio. At a waiting time of 800 fs, we observe population transfer from the B800 to B850 band as manifested by a prominent cross peak. These results will enable observation of the dynamics of biological systems across both ultrafast (<1 ps) and slower (>1 ms) timescales simultaneously.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Absorption
  • Bacterial Proteins / chemistry*
  • Lasers
  • Light-Harvesting Protein Complexes / chemistry*
  • Photons
  • Rhodobacter sphaeroides / enzymology
  • Spectrum Analysis / instrumentation
  • Spectrum Analysis / methods*

Substances

  • B800-850 light-harvesting complex, bacteria
  • Bacterial Proteins
  • Light-Harvesting Protein Complexes