Induction of apoptosis in human lymphoblastoid cells by kaempferol 3-O-β-isorhamninoside and rhamnocitrin 3-O-β-isorhamninoside from Rhamnus alaternus L. (Rhamnaceae)

Cell Prolif. 2011 Jun;44(3):283-90. doi: 10.1111/j.1365-2184.2011.00749.x.

Abstract

Objective: Kaempferol 3-O-β-isorhamninoside (K3O-ir) and rhamnocitrin 3-O-β-isorhamninoside (R3O-ir) from Rhamnus alaternus L leaves are investigated for their ability to induce apoptosis in human lymphoblastoid cells. We have attempted to characterize apoptotic pathway activated by these two flavonoids.

Material and methods: Apoptosis of the human TK6 lymphoblastoid cell line was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activity.

Results: Apoptosis was observed after 24- and 48-h incubation of the cells with the tested compounds. DNA fragmentation was observed after treatment with flavonoids; this was confirmed by demonstration of PARP cleavage. Caspase-3 and caspase-8 activities were induced by both K3O-ir and R3O-ir flavonoids showing highest activity with compound concentration of 400 μg/ml.

Conclusion: We have demonstrated that K3O-ir and R3O-ir induce apoptosis in human lymphoblastoid cells by the extrinsic pathway of apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis*
  • Caspase 3 / metabolism
  • Caspase 8 / metabolism
  • Caspases / metabolism
  • Cell Line
  • DNA Fragmentation
  • Enzyme Activation
  • Flavonols / chemistry*
  • Flavonols / pharmacology
  • Humans
  • Kaempferols / chemistry*
  • Kaempferols / pharmacology*
  • Lymphocytes / cytology*
  • Lymphocytes / drug effects*
  • Models, Chemical
  • Oxazines / pharmacology
  • Trisaccharides / pharmacology*
  • Xanthenes / pharmacology

Substances

  • Flavonols
  • Kaempferols
  • Oxazines
  • Trisaccharides
  • Xanthenes
  • kaempferol 3-O-isorhamninoside
  • kaempferol-7-methyl ether
  • resazurin
  • kaempferol
  • rhamnocitrin 3-O-isorhamninoside
  • Caspase 3
  • Caspase 8
  • Caspases