Robust in-gel fluorescence detection of mucin-type O-linked glycosylation

Balyn W Zaro; Leslie A Bateman; Matthew R Pratt

doi:10.1016/j.bmcl.2011.04.038

Robust in-gel fluorescence detection of mucin-type O-linked glycosylation

Bioorg Med Chem Lett. 2011 Sep 1;21(17):5062-6. doi: 10.1016/j.bmcl.2011.04.038. Epub 2011 Apr 14.

Authors

Balyn W Zaro¹, Leslie A Bateman, Matthew R Pratt

Affiliation

¹ Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA.

PMID: 21531554
DOI: 10.1016/j.bmcl.2011.04.038

Abstract

Mucin-type O-linked glycosylation is a common post translational modification of cell-surface and secretory pathway proteins and is implicated in vital biological processes as well as human disease. We report here the use of the metabolic chemical reporter GalNAz along with Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition conditions for the robust, in-gel fluorescent visualization of mucin-type O-linked glycoproteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Glycoproteins / chemistry
Glycosylation
Humans
Mucins / chemistry*
Spectrometry, Fluorescence

Substances

Glycoproteins
Mucins