Abstract
A quantitative method using artificial antibody to detect creatine kinases was developed. Linear epitope sequences were selected based on an artificial-epitope mapping strategy. Nine different MIPs corresponding to the selected peptides were then fabricated on QCM chips. The subtle conformational changes were also recognized by these chips.
MeSH terms
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Amino Acid Sequence
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Antibodies / immunology
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Creatine Kinase, MB Form / chemistry*
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Epitope Mapping / methods
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Molecular Imprinting / methods*
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Peptides / chemistry
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Polymers / chemistry
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Protein Isoforms / chemistry
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Protein Structure, Tertiary
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Quartz Crystal Microbalance Techniques
Substances
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Antibodies
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Peptides
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Polymers
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Protein Isoforms
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Creatine Kinase, MB Form