This work describes a bioassisted approach for the preparation of metal nanoparticles using yeast species Hansenula anomala. Gold nanoparticles were prepared using gold salt as the precursor, amine-terminated polyamidoamine dendrimer as the stabilizer, and the extracellular material from H. anomala as the bioreductant. It could also be demonstrated that, using our approach, small molecules such as cysteine can act as stabilizers as well. This synthetic approach offers a greener alternative route to the preparation of gold sols that are devoid of cellular and toxic chemical components. The ability of as-synthesized gold sol to function as biological ink for producing patterns for the analysis of fingerprints and to act as an antimicrobial reagent is evaluated. The generality of this toxin-free synthetic approach to other metals was assessed using palladium and silver.