Caenorhabditis elegans is a well-established model organism for the study of various biological areas. However, the procedures generally used for this worm are limited by the low throughput, low automation, and imprecise delivery of external stimuli. Microfluidics technology is emerging as an attractive and enabling platform to overcome these problems. In this review, we summarize the current microfluidic approaches for the investigation of behavior and neurobiology in C. elegans, and discuss the trends of future development.