Genetic expression of an amyloid peptide fragment and analysis of formylated products

G Cheng; C Krasel; H G Zhou; D Chappell; I W Hamley

doi:10.1021/ol1028145

Genetic expression of an amyloid peptide fragment and analysis of formylated products

Org Lett. 2011 May 20;13(10):2572-5. doi: 10.1021/ol1028145. Epub 2011 Apr 19.

Authors

G Cheng¹, C Krasel, H G Zhou, D Chappell, I W Hamley

Affiliation

¹ School of Chemistry, Food Science and Pharmacy, University of Reading, Whiteknights, Reading, UK.

PMID: 21504200
DOI: 10.1021/ol1028145

Abstract

The model amyloid peptide AAKLVFF was expressed as a His-tagged fusion protein with the immunoglobulin-binding domain B1 of streptococcal protein G (GB1), a small (56 residues), stable, single-domain protein. It is shown that expression of this model amyloid peptide is possible and is not hindered by aggregation. Formylation side reactions during the CNBr cleavage are investigated via synthesis of selectively formylated peptides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amyloid beta-Peptides* / chemistry
Amyloid beta-Peptides* / genetics
Amyloid beta-Peptides* / metabolism
Bacterial Proteins / metabolism
Click Chemistry
Formic Acid Esters / chemistry*
Genetic Engineering / methods
Histidine / chemistry
Models, Molecular*
Molecular Sequence Data
Molecular Structure
Peptide Fragments* / chemistry
Peptide Fragments* / genetics
Peptide Fragments* / metabolism

Substances

AAKLVFF
Amyloid beta-Peptides
Bacterial Proteins
Formic Acid Esters
IgG Fc-binding protein, Streptococcus
Peptide Fragments
Histidine