Abstract
Gene-targeted FLX titanium pyrosequencing integrated with stable isotope probing (SIP) using [(13)C]biphenyl substrate revealed that tidal mudflat sediments harbor novel aromatic ring hydroxylating dioxygenases (ARHD). More than 80% of the detected ARHD genes comprise four clades (0.5 distance) with 49 to 70% amino acid identity to sequences in public databases. The 16S rRNA sequences enriched in the (13)C fraction were from the Betaproteobacteria, bacilli (primarily Paenibacillus-like), and unclassified phyla.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria / classification
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Bacteria / enzymology*
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Bacteria / isolation & purification
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Bacteria / metabolism*
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Biphenyl Compounds / metabolism*
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Carbon Isotopes / metabolism
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Cluster Analysis
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Dioxygenases / genetics*
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Dioxygenases / metabolism
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Environmental Microbiology*
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Isotope Labeling
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Oxidation-Reduction
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Phylogeny
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Republic of Korea
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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Biphenyl Compounds
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Carbon Isotopes
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DNA, Bacterial
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diphenyl
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Dioxygenases