Silica nanoparticles (NPs) are biocompatible and well defined nanoporous material which can efficiently encapsulate drugs at high concentration, cross the cell membrane, and controllably release the cargo at the target site over a prescribed period of time. In the present study drug release and toxic behaviour of Mesoporous nanosilica was examined in vitro using dialysis membrane. Sol-gel method was used for the preparation of silica nanospheres. We have shown that fusion of a positively charged liposome with a negatively charged porous silica core synergistically loads and seals the drug within the core and can be useful for drug delivery.