Glycosidic bond cleavage of 5-fluoro-2'-deoxyuridine and 5-fluorouridine by amino acyl-tRNA synthetases

Biochem Biophys Res Commun. 1990 Dec 14;173(2):731-5. doi: 10.1016/s0006-291x(05)80096-8.

Abstract

Lysyl-tRNA synthetase isolated from rat liver cleaves glycosidic bond of 5-Fluorouridine and 5-Fluoro-2'-deoxyuridine to generate 5-Fluorouracil. The generation of 5-Fluorouracil was monitored by cellulose thin layer chromatography and by spectrophotometry. The enzyme was found to be 1.4 fold more efficient in cleaving the glycosidic bond of 5-Fluoro-2'-deoxyuridine than 5-Fluorouridine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Thin Layer
  • Cytoplasm / enzymology
  • Floxuridine / metabolism*
  • Glycoside Hydrolases / metabolism
  • Glycosides / metabolism*
  • Hydrolysis
  • Liver / enzymology
  • Lysine-tRNA Ligase / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Uridine / analogs & derivatives*
  • Uridine / metabolism

Substances

  • Glycosides
  • Floxuridine
  • 5-fluorouridine
  • Glycoside Hydrolases
  • Lysine-tRNA Ligase
  • doxifluridine
  • Uridine