Background: The well-known chemically and therapeutically active compound allicin is formed in crushed garlic by the interaction of alliin with alliinase. In this study, low-frequency and low-intensity ultrasound was employed to accelerate the alliinase-catalysed synthesis of allicin in freshly crushed garlic.
Results: The optimal conditions for improvement of the alliinase-catalysed synthesis of allicin in freshly crushed garlic were found to be as follows: ultrasound intensity 0.4 W cm⁻², ultrasound frequency 50 kHz, enzymatic reaction temperature 35 °C and reaction time 30 min. Under these conditions the yield of allicin was increased by about 25.2% compared with the control without ultrasound. Alliinase in the freshly crushed garlic was purified by ammonium sulfate precipitation and gel filtration on a Sephacryl S-200 column. The employed ultrasound increased the activity of the purified alliinase by about 42.8%, did not affect the enzyme's temperature optimum and improved its thermal stability.
Conclusion: The results of this study indicated that the activity of alliinase in freshly crushed garlic might be enhanced by low-frequency and low-intensity ultrasound, thereby accelerating the alliinase-catalysed conversion of alliin in garlic to allicin.
Copyright © 2011 Society of Chemical Industry.