Compensation-free, all-fiber-optic, two-photon endomicroscopy at 1.55 μm

Opt Lett. 2011 Apr 1;36(7):1299-301. doi: 10.1364/OL.36.001299.

Abstract

We present an all-fiber-optic scanning multiphoton endomicroscope with 1.55 μm excitation without the need for prechirping femtosecond pulses before the endomicroscope. The system consists of a 1.55 μm femtosecond fiber laser, a customized double-clad fiber for light delivery and fluorescence collection, and a piezoelectric scan head. We demonstrate two-photon imaging of cultured cells and mouse tissue, both labeled with indocyanine green. Free-space multiphoton imaging with near-IR emission has previously shown benefits in reduced background fluorescence and lower attenuation for the fluorescence emission. For fiber-optic multiphoton imaging there is the additional advantage of using the soliton effect at the telecommunication wavelengths (1.3-1.6 μm) in fibers, permitting dispersion-compensation-free, small-footprint systems. We expect these advantages will help transition multiphoton endomicroscopy to the clinic.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bile Acids and Salts / metabolism
  • Cell Line
  • Infrared Rays
  • Mice
  • Microscopy / instrumentation*
  • Optical Fibers*
  • Photons*

Substances

  • Bile Acids and Salts