Noninvasive bioluminescent quantification of viable stem cells in engineered constructs

Methods Mol Biol. 2011:740:165-78. doi: 10.1007/978-1-61779-108-6_18.

Abstract

Bioluminescence from murine stem cells tagged with the luciferase gene reporter and distributed within three-dimensional scaffolds of two different materials is quantified in vitro and in vivo. The luminescence emitted from cells adhering to the scaffolds tested is monitored noninvasively using a bioluminescence imaging system. Monitoring the kinetics of luciferase expression via bioluminescence enables real-time assessment of cell survival and proliferation on scaffolds both in vitro and in vivo over prolonged (8 weeks) periods of time.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation
  • Cell Survival
  • Imaging, Three-Dimensional
  • Implants, Experimental
  • Luminescent Measurements / methods*
  • Mesenchymal Stem Cell Transplantation
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / metabolism*
  • Mice
  • Mice, Nude
  • Time Factors
  • Tissue Engineering / methods*
  • Tissue Scaffolds / chemistry*
  • Whole Body Imaging