Abstract
hnRNP K protein is a member of the heterogeneous nuclear protein (hnRNP) complex that, besides its function as a translational regulator of human mRNA, is also considered to be a transcription factor involved in tumorigenesis. PSF is a protein part of the human spliceosome and essential in RNA splicing. Here we report the generation of one monoclonal antibody GG6H9.1C3 that recognized both hnRNP K and PSF proteins using Western blot analysis, flow cytometry, and immunocytochemistry.
MeSH terms
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Antibodies, Monoclonal / immunology*
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Blotting, Western
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Cell Nucleus / metabolism
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Flow Cytometry
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Heterogeneous-Nuclear Ribonucleoprotein K / immunology*
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Heterogeneous-Nuclear Ribonucleoprotein K / metabolism
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Humans
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Immunohistochemistry
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PTB-Associated Splicing Factor
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RNA-Binding Proteins / immunology*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Antibodies, Monoclonal
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Heterogeneous-Nuclear Ribonucleoprotein K
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PTB-Associated Splicing Factor
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RNA-Binding Proteins