Supplementation with estradiol-17β improves porcine oocyte maturation and subsequent embryo development

Fertil Steril. 2011 Jun 30;95(8):2582-4. doi: 10.1016/j.fertnstert.2011.03.013. Epub 2011 Apr 3.

Abstract

Metaphase II oocyte production was significantly increased by treatment with E(2) during the first half of the total in vitro maturation (IVM) period, which was further evidenced by an increase in monospermic fertilization, blastocyst formation, or blastomere viability of IVF- or somatic cell nuclear transfer-derived embryos. Thus, we concluded that transient E(2) supplementation could improve the IVM rate and subsequent developmental competence in pigs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst / drug effects*
  • Blastocyst / physiology
  • CDC2 Protein Kinase / metabolism
  • Cells, Cultured
  • Embryo Culture Techniques
  • Embryonic Development / drug effects
  • Estradiol / pharmacology*
  • Female
  • Fertilization in Vitro*
  • Meiosis / drug effects
  • Mitogen-Activated Protein Kinase 1 / metabolism
  • Mitogen-Activated Protein Kinase 3 / metabolism
  • Nuclear Transfer Techniques*
  • Oocytes / drug effects*
  • Oocytes / physiology
  • Phosphorylation
  • Swine
  • Time Factors

Substances

  • Estradiol
  • CDC2 Protein Kinase
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3