Abstract
We assessed the microbiota of a tongue abscess in which twelve different aerobic and anaerobic bacteria were identified using fluorescent in situ hybridisation (FISH), sequencing of the 16S rRNA gene and phenotypic methods. By applying the 16S rRNA based probes directly on the clinical material, a quick insight of the bacteria present was obtained and the species which were not cultured but present in the abscess were identified.
Copyright © 2011 Elsevier Ltd. All rights reserved.
MeSH terms
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Abscess / microbiology*
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Abscess / pathology
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Adolescent
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Bacteria / classification*
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Bacteria / genetics
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Bacteria / isolation & purification*
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Bacteria / metabolism
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Bacterial Infections / microbiology*
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Bacterial Infections / pathology
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Bacterial Typing Techniques / methods
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Bacteriological Techniques / methods*
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Female
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Humans
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In Situ Hybridization, Fluorescence / methods
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RNA, Bacterial / genetics
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA / methods
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Tongue Diseases / microbiology*
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Tongue Diseases / pathology
Substances
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RNA, Bacterial
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RNA, Ribosomal, 16S