Abstract
N-Terminal pro-brain natriuretic peptide (NT-proBNP) is an important marker for heart failure that reflects ventricular volume expansion, ventricular overload and the degree of cardiac injury. To establish a sensitive and simple detection method for it in serum, a regeneration-free immunosensor with novel Fab fragment monoclonal antibodies was prepared. The sensor detected NT-proBNP from 0.04 to 2.5 ng/ml with a limit of 0.03 ng/ml. The immunosensor is therefore a simple, cost-effective method to detect NT-proBNP and the proposed immunoassay system would enable other proteins to be detected and open new opportunities for protein diagnostics.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / chemistry*
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Antibodies, Monoclonal / immunology*
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Biomarkers / analysis
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Biosensing Techniques / methods
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Electrochemical Techniques / methods*
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Heart Failure / metabolism
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Heart Failure / pathology
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Humans
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Immunoassay / methods*
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Immunoglobulin Fab Fragments / chemistry*
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Immunoglobulin Fab Fragments / immunology*
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Immunohistochemistry
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Magnetite Nanoparticles
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Natriuretic Peptide, Brain / blood
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Natriuretic Peptide, Brain / immunology*
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Peptide Fragments / blood
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Peptide Fragments / immunology*
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Recombinant Proteins
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Regression Analysis
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Sensitivity and Specificity
Substances
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Antibodies, Monoclonal
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Biomarkers
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Immunoglobulin Fab Fragments
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Magnetite Nanoparticles
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Peptide Fragments
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Recombinant Proteins
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pro-brain natriuretic peptide (1-76)
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Natriuretic Peptide, Brain