Abstract
Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cluster Analysis
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Humans
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Internet
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Molecular Sequence Data
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Orbivirus / isolation & purification*
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Phylogeny
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RNA, Viral / genetics
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RNA-Dependent RNA Polymerase / genetics
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Reoviridae Infections / diagnosis*
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Reoviridae Infections / veterinary*
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Viral Proteins / genetics
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Virology / methods*
Substances
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RNA, Viral
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Viral Proteins
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RNA-Dependent RNA Polymerase
Associated data
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