Coffees rich in chlorogenic acid or N-methylpyridinium induce chemopreventive phase II-enzymes via the Nrf2/ARE pathway in vitro and in vivo

Mol Nutr Food Res. 2011 May;55(5):798-802. doi: 10.1002/mnfr.201100115. Epub 2011 Mar 24.

Abstract

Recently, the coffee constituents 5-O-caffeoylquinic acid (CGA) and N-methylpyridinium (NMP) were identified as inducers of the Nrf2/antioxidant-response element (ARE) detoxifying pathway under cell-culture condition. To study the impact of CGA and NMP on the Nrf2-activating properties of a complex coffee beverage, two different model coffees were generated by variation of the roasting conditions: a low-roast coffee rich in CGA and a heavy-roast low in CGA but containing high levels of NMP. Activation of the Nrf2/antioxidant-response element pathway was monitored in vitro and in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Chemoprevention*
  • Chlorogenic Acid / pharmacology*
  • Coffee / chemistry*
  • Enzyme Induction / drug effects
  • Glutamate-Cysteine Ligase / biosynthesis
  • HT29 Cells
  • Heme Oxygenase-1 / biosynthesis
  • Humans
  • NF-E2-Related Factor 2 / physiology*
  • Pyridinium Compounds / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Response Elements / physiology*

Substances

  • Antioxidants
  • Coffee
  • NF-E2-Related Factor 2
  • NFE2L2 protein, human
  • Pyridinium Compounds
  • Reactive Oxygen Species
  • Chlorogenic Acid
  • 1-methylpyridinium
  • HMOX1 protein, human
  • Heme Oxygenase-1
  • Glutamate-Cysteine Ligase