Quantitative detection of adenosine in urine using silver enhancement of aptamer-gold nanoparticle aggregation and progressive dilution

Zhao Fang Liu; Jing Ge; Xin Sheng Zhao

doi:10.1039/c1cc10460h

Quantitative detection of adenosine in urine using silver enhancement of aptamer-gold nanoparticle aggregation and progressive dilution

Chem Commun (Camb). 2011 May 7;47(17):4956-8. doi: 10.1039/c1cc10460h. Epub 2011 Mar 28.

Authors

Zhao Fang Liu¹, Jing Ge, Xin Sheng Zhao

Affiliation

¹ Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

PMID: 21445394
DOI: 10.1039/c1cc10460h

Abstract

We implement the progressive dilution strategy to bring assays based on gold nanoparticles to a quantitative level. This is demonstrated by the detection of adenosine in urine by combining progressive dilution with the silver enhancement of aptamer-gold nanoparticle aggregation, giving good accuracy, high selectivity, and an unlimited dynamic range above LOD.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine / urine*
Aptamers, Nucleotide / chemical synthesis
Aptamers, Nucleotide / metabolism*
Biosensing Techniques*
Gold / chemistry
Gold / metabolism
Humans
Indicator Dilution Techniques
Limit of Detection
Metal Nanoparticles / chemistry
Sensitivity and Specificity
Silver / chemistry
Silver / metabolism

Substances

Aptamers, Nucleotide
Silver
Gold
Adenosine