Localization and activity levels of the following digestive enzymes in the intestine of free-living large-scaled gurnard were determined: non-specific esterase, alkaline and acid phosphatase as well as aminopeptidase. Enzymatic activity of the four enzymes was confirmed in all intestine parts but with different distribution through the enterocytes and varying in diverse intensity according to intestine part. This research is part of a broader research project on the biology of economically important fish from the Adriatic Sea. Our study reveals that in the large-scaled gurnard, the middle and posterior intestinal segments play the major role in digestion and absorption of proteins, whereas all parts of the intestine participate in lipid absorption and intracellular digestion. The high protein and lipid content in the diet of the large-scaled gurnard is most likely responsible for high activities of esterase, alkaline phosphatase and aminopeptidase, as they are involved in digestion and absorption of proteins and lipids.
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