The purpose of the study was to determine the capability of bioimpedance spectroscopy (BIS) to evaluate the hematological and biochemical parameters of blood. BIS readings were estimated on an ABC-01 Medass hardware-computer complex; blood hematological and biochemical parameters (n = 46) were determined on automated analyzers. The BIS readings were found to be closely correlated with packed cell volume and hemoglobin and red blood cell values, but weakly related to plasma biochemical parameters and white blood cell counts. BIS readings may be used to calculate red blood cell counts taken in units of measurement in hematological practice, by applying the multiple regression equations. Thus, the blood BIS technique may be employed to estimate basic hematological parameters (hemoglobin concentration, red blood cell counts, and packed cell volume).