[An application of oligonucleotide-directed mutagenesis on making deletion mutation]

N Wei; N Qin; Y Y Li; S M Wu

[An application of oligonucleotide-directed mutagenesis on making deletion mutation]

Yi Chuan Xue Bao. 1990;17(1):63-9.

[Article in Chinese]

Authors

N Wei¹, N Qin, Y Y Li, S M Wu

Affiliation

¹ Institute of Genetics, Fudan University, Shanghai.

PMID: 2142602

Abstract

By the method of oligonucleotide-directed mutagenesis, we made 27bp and 18bp deletion at junction region between alpha-factor signal sequence and alpha-hANP gene, alpha-factor signal sequence and alpha-IFN gene, respectively. Since the deleted region contains one HindIII site, the mutants without this site were selected. The result of DNA sequence analysis showed that the sequences of the mutants were the same as designed.

Publication types

English Abstract

MeSH terms

Atrial Natriuretic Factor / genetics
Base Sequence
Chromosome Deletion*
Interferon Type I / genetics
Molecular Sequence Data
Mutation*
Oligonucleotides / pharmacology*
Peptide Fragments / genetics

Substances

Interferon Type I
Oligonucleotides
Peptide Fragments
Atrial Natriuretic Factor