Surface modification and biomolecule immobilization on polymer spheres for biosensing applications

Chris R Taitt; Lisa C Shriver-Lake; George P Anderson; Frances S Ligler

doi:10.1007/978-1-61779-052-2_6

Surface modification and biomolecule immobilization on polymer spheres for biosensing applications

Methods Mol Biol. 2011:726:77-94. doi: 10.1007/978-1-61779-052-2_6.

Authors

Chris R Taitt¹, Lisa C Shriver-Lake, George P Anderson, Frances S Ligler

Affiliation

¹ Center for Bio/Molecular Science and Engineering, U.S. Naval Research Laboratory, Washington, DC, USA.

PMID: 21424444
DOI: 10.1007/978-1-61779-052-2_6

Abstract

Microspheres and nanospheres are being used in many of today's biosensing applications for automated sample processing, flow cytometry, signal amplification in microarrays, and labeling in multiplexed analyses. The surfaces of the spheres/particles need to be modified with proteins and other biomolecules to be used in these sensing applications. This chapter contains protocols to modify carboxyl- and amine-coated polymer spheres with proteins and peptides.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Antimicrobial Cationic Peptides / metabolism
Biosensing Techniques / instrumentation
Biosensing Techniques / methods*
Fumonisins / chemistry
Fumonisins / metabolism
Immunoassay
Microspheres*
Nanotechnology
Ochratoxins / chemistry
Ochratoxins / metabolism
Polymers / chemistry*
Polymers / metabolism*
Proteins / metabolism
Surface Properties

Substances

Antimicrobial Cationic Peptides
Fumonisins
Ochratoxins
Polymers
Proteins
ochratoxin A
fumonisin B1