A label-free DNA reduced graphene oxide-based fluorescent sensor for highly sensitive and selective detection of hemin

Chem Commun (Camb). 2011 Apr 28;47(16):4676-8. doi: 10.1039/c0cc05518b. Epub 2011 Mar 21.

Abstract

G-quadruplex structure aptamer (PS2.M) can capture acridine orange (AO) from reduced graphene oxide (rGO). When the AO-PS2.M/rGO mixture is incubated with hemin, the specific binding of hemin with PS2.M results in a release of AO from PS2.M and return of AO back to rGO. Based on the quenching of fluorescence, the target hemin was detected sensitively and selectively, giving a detection limit of 50 nM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acridine Orange / chemistry*
  • Aptamers, Nucleotide / chemistry
  • DNA / chemistry*
  • Fluorescent Dyes / chemistry*
  • G-Quadruplexes
  • Graphite / chemistry*
  • Hemin / analysis*
  • Molecular Structure
  • Oxidation-Reduction
  • Oxides / chemistry*

Substances

  • Aptamers, Nucleotide
  • Fluorescent Dyes
  • Oxides
  • Hemin
  • Graphite
  • DNA
  • Acridine Orange