Bioflocculant production by Virgibacillus sp. Rob isolated from the bottom sediment of Algoa Bay in the Eastern Cape, South Africa

Sekelwa Cosa; Leonard V Mabinya; Ademola O Olaniran; Omobola O Okoh; Kim Bernard; Shaun Deyzel; Anthony I Okoh

doi:10.3390/molecules16032431

Bioflocculant production by Virgibacillus sp. Rob isolated from the bottom sediment of Algoa Bay in the Eastern Cape, South Africa

Molecules. 2011 Mar 14;16(3):2431-42. doi: 10.3390/molecules16032431.

Authors

Sekelwa Cosa¹, Leonard V Mabinya, Ademola O Olaniran, Omobola O Okoh, Kim Bernard, Shaun Deyzel, Anthony I Okoh

Affiliation

¹ Applied and Environmental Microbiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.

Abstract

A bioflocculant-producing marine bacterium previously isolated from marine sediment of Algoa Bay was screened for flocculant production. Comparative analysis of 16S rDNA sequence identified the isolate to have 99% similarity to Virgibacillus sp. XQ-1 and it was deposited in the GenBank as Virgibacillus sp. Rob with accession number HQ537127. The bacterium produced biflocculants optimally in glucose (70.4%) and peptone (70.4%) as sole sources of carbon and nitrogen, alkaline pH (12) (74%); and the presence of Fe2+ (74%). Chemical analysis of the bioflocculant revealed it to be a polysaccharide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA Primers
Flocculation
Geologic Sediments*
Hydrogen-Ion Concentration
Molecular Sequence Data
South Africa
Virgibacillus / genetics
Virgibacillus / isolation & purification
Virgibacillus / metabolism*
Water Microbiology*

Substances

DNA Primers

Associated data

GENBANK/HQ537127