SYBR Green I-based real-time PCR targeting the rpoX gene for sensitive and rapid detection of Vibrio alginolyticus

Mol Cell Probes. 2011 Apr-Jun;25(2-3):137-41. doi: 10.1016/j.mcp.2011.02.002. Epub 2011 Mar 21.

Abstract

rpoX, a Vibrio alginolyticus specific stress regulating gene, was used to detect this fish pathogen by SYBR Green I-based real-time PCR. The specificity of the detection was confirmed in different samples. The minimum level of detection was 10(3) cells from pure culture and 10(2) cells from seawater.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / genetics*
  • Benzothiazoles
  • DNA Primers / genetics
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Diamines
  • Fish Diseases / diagnosis
  • Fish Diseases / microbiology*
  • Organic Chemicals / chemistry*
  • Perciformes
  • Polymerase Chain Reaction / methods*
  • Quinolines
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Sigma Factor / genetics
  • Vibrio alginolyticus / genetics*

Substances

  • Bacterial Proteins
  • Benzothiazoles
  • DNA Primers
  • DNA, Bacterial
  • Diamines
  • Organic Chemicals
  • Quinolines
  • Sigma Factor
  • SYBR Green I