Abstract
rpoX, a Vibrio alginolyticus specific stress regulating gene, was used to detect this fish pathogen by SYBR Green I-based real-time PCR. The specificity of the detection was confirmed in different samples. The minimum level of detection was 10(3) cells from pure culture and 10(2) cells from seawater.
Copyright © 2011 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / genetics*
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Benzothiazoles
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DNA Primers / genetics
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Diamines
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Fish Diseases / diagnosis
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Fish Diseases / microbiology*
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Organic Chemicals / chemistry*
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Perciformes
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Polymerase Chain Reaction / methods*
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Quinolines
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Reproducibility of Results
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Sensitivity and Specificity
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Sigma Factor / genetics
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Vibrio alginolyticus / genetics*
Substances
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Bacterial Proteins
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Benzothiazoles
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DNA Primers
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DNA, Bacterial
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Diamines
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Organic Chemicals
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Quinolines
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Sigma Factor
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SYBR Green I