Abstract
Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1,4-benzoquinone) for immunoflourescence staining of neurospheres.
Copyright © 2011 Elsevier B.V. All rights reserved.
MeSH terms
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AC133 Antigen
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Animals
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Antigens, CD / metabolism*
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Benzoquinones / pharmacology
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Fixatives / pharmacology
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Glioblastoma / pathology
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Glycoproteins / metabolism*
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Intermediate Filament Proteins / metabolism
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Mice
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Neoplastic Stem Cells / drug effects
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Neoplastic Stem Cells / metabolism*
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Neoplastic Stem Cells / pathology*
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Nerve Tissue Proteins / metabolism
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Nestin
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Peptides / metabolism*
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Tumor Cells, Cultured
Substances
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AC133 Antigen
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Antigens, CD
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Benzoquinones
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Fixatives
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Glycoproteins
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Intermediate Filament Proteins
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Nerve Tissue Proteins
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Nes protein, mouse
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Nestin
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Peptides
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Prom1 protein, mouse
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quinone