LNA for optimization of fluorescent oligonucleotide probes: improved spectral properties and target binding

Irina V Astakhova; Alexey V Ustinov; Vladimir A Korshun; Jesper Wengel

doi:10.1021/bc1005027

LNA for optimization of fluorescent oligonucleotide probes: improved spectral properties and target binding

Bioconjug Chem. 2011 Apr 20;22(4):533-9. doi: 10.1021/bc1005027. Epub 2011 Mar 14.

Authors

Irina V Astakhova¹, Alexey V Ustinov, Vladimir A Korshun, Jesper Wengel

Affiliation

¹ Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark , DK-5230 Odense M, Denmark.

PMID: 21401111
DOI: 10.1021/bc1005027

Abstract

Mixmer LNA/DNA fluorescent probes containing the 1-(phenylethynyl)pyrene fluorophore attached to 2'-arabino-uridine were synthesized and studied. The conjugates displayed significantly higher hybridization affinity to target DNA, increased fluorescence quantum yields of single-stranded oligonucleotides and their duplexes, and improved ability to form an interstrand excimer compared to analogous non-LNA probes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites
DNA / chemistry*
DNA / metabolism
Fluorescence*
Molecular Conformation
Oligonucleotide Probes / chemistry*
Oligonucleotide Probes / metabolism
Oligonucleotides / chemistry*
Oligonucleotides / metabolism

Substances

Oligonucleotide Probes
Oligonucleotides
locked nucleic acid
DNA