c-di-GMP can form remarkably stable G-quadruplexes at physiological conditions in the presence of some planar intercalators

Shizuka Nakayama; Ilana Kelsey; Jingxin Wang; Herman O Sintim

doi:10.1039/c0cc05432a

c-di-GMP can form remarkably stable G-quadruplexes at physiological conditions in the presence of some planar intercalators

Chem Commun (Camb). 2011 Apr 28;47(16):4766-8. doi: 10.1039/c0cc05432a. Epub 2011 Mar 14.

Authors

Shizuka Nakayama¹, Ilana Kelsey, Jingxin Wang, Herman O Sintim

Affiliation

¹ Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.

PMID: 21399808
DOI: 10.1039/c0cc05432a

Abstract

The ubiquitous bacterial biofilm regulator, c-di-GMP can form G-quadruplexes at physiological conditions in the presence of some aromatic compounds, such as acriflavine and proflavine. The fluorescence of these compounds is quenched upon c-di-GMP binding and some of the formed c-di-GMP G-quadruplexes are stable even at 75 °C.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acriflavine / chemistry*
Cyclic GMP / analogs & derivatives*
Cyclic GMP / chemistry
Fluorescence
G-Quadruplexes*
Proflavine / chemistry*
Temperature

Substances

Acriflavine
bis(3',5')-cyclic diguanylic acid
Proflavine
Cyclic GMP