Abstract
We studied the induction of antigen-specific IgA memory B cells (B(M)) in volunteers who received live attenuated Shigella flexneri 2a vaccines. Subjects ingested a single oral dose of 10(7), 10(8) or 10(9) CFU of S. flexneri 2a with deletions in guaBA (CVD 1204) or in guaBA, set and sen (CVD 1208). Antigen-specific serum and stool antibody responses to LPS and Ipa B were measured on days 0, 7, 14, 28 and 42. IgA B(M) cells specific to LPS, Ipa B and total IgA were assessed on days 0 and 28. We show the induction of significant LPS-specific IgA B(M) cells in anti-LPS IgA seroresponders. Positive correlations were found between anti-LPS IgA B(M) cells and anti-LPS IgA in serum and stool; IgA B(M) cell responses to IpaB were also observed. These B(M) cell responses are likely play an important role in modulating the magnitude and longevity of the humoral response.
Copyright © 2011 Elsevier Inc. All rights reserved.
Publication types
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Clinical Trial
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Research Support, N.I.H., Extramural
MeSH terms
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Administration, Oral
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Adolescent
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Adult
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Antibody Formation / immunology
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Antigens / immunology*
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Antigens, CD / metabolism
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B-Lymphocyte Subsets / cytology
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B-Lymphocyte Subsets / immunology*
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B-Lymphocyte Subsets / metabolism
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Bacterial Proteins / immunology
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Feces / chemistry
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Humans
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Immunity, Mucosal / immunology
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Immunoglobulin A / analysis
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Immunoglobulin A / blood
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Immunoglobulin A / immunology*
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Immunoglobulin A / metabolism
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Immunoglobulin G / blood
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Immunoglobulin G / immunology
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Immunoglobulin G / metabolism
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Integrins / metabolism
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / immunology
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Lipopolysaccharides / immunology
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Lymphocyte Count
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Middle Aged
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Sequence Deletion
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Shigella Vaccines / administration & dosage
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Shigella Vaccines / immunology*
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Shigella flexneri / genetics
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Shigella flexneri / immunology*
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Vaccination / methods*
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Vaccines, Attenuated / administration & dosage
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Vaccines, Attenuated / immunology*
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Young Adult
Substances
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Antigens
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Antigens, CD
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Bacterial Proteins
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Immunoglobulin A
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Immunoglobulin G
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Integrins
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Lipopolysaccharides
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Shigella Vaccines
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Vaccines, Attenuated
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integrin alpha4beta7
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ipaB protein, Shigella