Abstract
Deletion of Mycobacterium marinum MMAR2333 resulted in the loss of three of four subclasses of lipooligosaccharides (LOSs). The mutant was unable to extend an intermediate (LOS-II*) by addition of caryophyllose. These data and the predicted domain structure suggest that MMAR2333 is a glycosyltransferase involved in the generation of a lipid-linked caryophyllose donor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Carbohydrates
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Cell Wall / metabolism
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Gene Expression Regulation, Bacterial / physiology*
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Gene Expression Regulation, Enzymologic / physiology*
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Glycosyltransferases / genetics
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Glycosyltransferases / metabolism*
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Lipopolysaccharides / metabolism*
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Models, Molecular
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Molecular Sequence Data
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Mutation
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Mycobacterium marinum / enzymology*
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Mycobacterium marinum / genetics
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Protein Conformation
Substances
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Bacterial Proteins
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Carbohydrates
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Lipopolysaccharides
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lipid-linked oligosaccharides
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Glycosyltransferases