Construction and application of a functional library of cytochrome P450 monooxygenases from the filamentous fungus Aspergillus oryzae

Appl Environ Microbiol. 2011 May;77(9):3147-50. doi: 10.1128/AEM.02491-10. Epub 2011 Mar 4.

Abstract

A functional library of cytochrome P450 monooxygenases from Aspergillus oryzae (AoCYPs) was constructed in which 121 isoforms were coexpressed with yeast NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae. Using this functional library, novel catalytic functions of AoCYPs, such as catalytic potentials of CYP57B3 against genistein, were elucidated for the first time. Comprehensive functional screening promises rapid characterization of catalytic potentials and utility of AoCYPs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus oryzae / enzymology*
  • Aspergillus oryzae / genetics*
  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / metabolism*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Expression
  • Genistein / metabolism
  • Metabolic Networks and Pathways / genetics
  • Oxidation-Reduction
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics
  • Substrate Specificity

Substances

  • Fungal Proteins
  • Recombinant Proteins
  • Cytochrome P-450 Enzyme System
  • Genistein