Abstract
The role of exocytosis of major histocompatibility complex (MHC) class I molecules in the presentation of antigens to mouse cytotoxic T lymphocytes (CTLs) was examined by use of a recombinant vaccinia virus that expresses the E19 glycoprotein from adenovirus. E19 blocked the presentation of vaccinia and influenza virus proteins to CTLs in a MHC class I allele-specific manner identical to its inhibition of MHC class I transport from the endoplasmic reticulum. This finding indicates that (i) the relevant parameter for antigen presentation is the rate of MHC class I molecule exocytosis, not the level of class I cell surface expression, and (ii) association of class I molecules with antigen is likely to occur within the endoplasmic reticulum.
MeSH terms
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Adenovirus Early Proteins
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Animals
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Antigen-Presenting Cells / drug effects
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Antigen-Presenting Cells / immunology
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Antigen-Presenting Cells / ultrastructure*
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Antigens / immunology*
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Antigens, Viral / immunology
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Antiviral Agents
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Biological Transport
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Brefeldin A
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Cell Line
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Cyclopentanes / pharmacology
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Endoplasmic Reticulum / immunology*
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Exocytosis
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Glycoproteins / analysis
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H-2 Antigens / immunology
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Histocompatibility Antigens Class I / immunology
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Histocompatibility Antigens Class I / metabolism*
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Mice
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Mice, Inbred A
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Mice, Inbred BALB C
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Mice, Inbred C3H
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Mice, Inbred CBA
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Oncogene Proteins, Viral / analysis
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Orthomyxoviridae Infections / immunology
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Recombinant Proteins / immunology
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T-Lymphocytes, Cytotoxic / immunology*
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Vaccinia virus / immunology
Substances
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Adenovirus Early Proteins
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Antigens
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Antigens, Viral
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Antiviral Agents
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Cyclopentanes
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Glycoproteins
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H-2 Antigens
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Histocompatibility Antigens Class I
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Oncogene Proteins, Viral
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Recombinant Proteins
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Brefeldin A