Anti-inflammatory diterpene from Thyrsanthera suborbicularis

Chem Pharm Bull (Tokyo). 2011;59(3):382-4. doi: 10.1248/cpb.59.382.

Abstract

Bioactivity-guided isolation on a n-hexane-soluble fraction of Thyrsanthera suborbicularis led to the isolation of a new rosane-type diterpene, 19-hydroxy-1(10), 15-rosadiene (1), along with three known compounds, taraxerol, acetyl aleuritolic acid, and spathulenol. The structures of isolated compounds were determined by interpretation of NMR spectroscopic data and mass spectrometry. Compound 1 demonstrated significantly inhibitory activity on nitric oxide production in RAW264.7 lipopolysaccharide (LPS)-activated mouse macrophages with an IC(50) value of 2.91 µg/ml via the suppression of inducible nitric oxide synthase (iNOS) mRNA expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry*
  • Anti-Inflammatory Agents / isolation & purification
  • Cell Line, Tumor
  • Diterpenes / chemistry*
  • Diterpenes / isolation & purification
  • Euphorbiaceae / chemistry*
  • Lipopolysaccharides / toxicity
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Mice
  • Molecular Conformation
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase Type II / genetics
  • Nitric Oxide Synthase Type II / metabolism
  • RNA, Messenger / metabolism

Substances

  • Anti-Inflammatory Agents
  • Diterpenes
  • Lipopolysaccharides
  • RNA, Messenger
  • Nitric Oxide
  • Nitric Oxide Synthase Type II