Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216

Liyan Li; Xiaolu Jiang; Huashi Guan; Peng Wang

doi:10.1016/j.carres.2011.01.023

Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216

Carbohydr Res. 2011 May 1;346(6):794-800. doi: 10.1016/j.carres.2011.01.023. Epub 2011 Jan 28.

Authors

Liyan Li¹, Xiaolu Jiang, Huashi Guan, Peng Wang

Affiliation

¹ School of Medicine and Pharmaceutics, Ocean University of China, Qingdao 266003, PR China.

PMID: 21371694
DOI: 10.1016/j.carres.2011.01.023

Abstract

Alginate lyase which was purified from the fermentation solution of marine bacteria Pseudomonas sp. HJZ216 was applied to hydrolyze algae alginate. Six oligosaccharides, including di- and trisaccharides, were isolated and purified through anion exchange chromatography. The oligosaccharide structures were elucidated based on electrospray ionization-mass spectrometry (ESI-MS) and 2D NMR spectra analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alginates / chemistry*
Glucuronic Acid / chemistry
Hexuronic Acids / chemistry
Oligosaccharides / chemistry
Oligosaccharides / isolation & purification*
Oligosaccharides / metabolism*
Polysaccharide-Lyases / metabolism*
Pseudomonas / enzymology*

Substances

Alginates
Hexuronic Acids
Oligosaccharides
Glucuronic Acid
Polysaccharide-Lyases
poly(beta-D-mannuronate) lyase