Improving sensitivity in nonlinear Raman microspectroscopy imaging and sensing

J Biomed Opt. 2011 Feb;16(2):021114. doi: 10.1117/1.3533317.

Abstract

Nonlinear Raman microspectroscopy based on a broadband coherent anti-Stokes Raman scattering is an emerging technique for noninvasive, chemically specific, microscopic analysis of tissues and large population of cells and particles. The sensitivity of this imaging is a critical aspect of a number of the proposed biomedical application. It is shown that the incident laser power is the major parameter controlling this sensitivity. By careful optimizing the laser system, the high-quality vibrational spectra acquisition at the multi-kHz rate becomes feasible.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Computer-Aided Design
  • Equipment Design
  • Equipment Failure Analysis
  • Image Enhancement / instrumentation*
  • Lasers*
  • Microscopy / instrumentation*
  • Nonlinear Dynamics
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrum Analysis, Raman / instrumentation*