Sequence complementarity-driven nonenzymatic ligation of RNA

Samanta Pino; Giovanna Costanzo; Alessandra Giorgi; Ernesto Di Mauro

doi:10.1021/bi101981z

Sequence complementarity-driven nonenzymatic ligation of RNA

Biochemistry. 2011 Apr 12;50(14):2994-3003. doi: 10.1021/bi101981z. Epub 2011 Mar 16.

Authors

Samanta Pino¹, Giovanna Costanzo, Alessandra Giorgi, Ernesto Di Mauro

Affiliation

¹ Dipartimento di Biologia e Biotecnologie Charles Darwin, Sapienza Università di Roma, P.le Aldo Moro, 5, Rome 00185, Italy.

PMID: 21361363
DOI: 10.1021/bi101981z

Abstract

We report two reactions of RNA G:C sequences occurring nonenzymatically in water in the absence of any added cofactor or metal ion: (a) sequence complementarity-driven terminal ligation and (b) complementary sequence adaptor-driven multiple tandemization. The two abiotic reactions increase the chemical complexity of the resulting pool of RNA molecules and change the Shannon information of the initial population of sequences.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Pairing
Base Sequence
Cyclic AMP / chemistry
Cyclic GMP / chemistry
Kinetics
Models, Chemical*
Molecular Structure
Nucleic Acid Conformation*
Oligoribonucleotides / chemistry*
Oligoribonucleotides / genetics
RNA / chemistry*
RNA / genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Water / chemistry

Substances

Oligoribonucleotides
Water
RNA
Cyclic AMP
Cyclic GMP