Publisher in February 2008 WHO M-POWER report indicates that every year on the world tobacco epidemics kills 5.4 million of people and the analysis of tobacco smoking prevalence change shows great differences between European countries. It is estimated that in Poland 29% of adult people smokes, and 24% in Slovakia. However tobacco smoking among academic youth is still a big problem. The aim of the study was an attempt to estimate tobacco smoking prevalence among students from Poland and Slovakia. The study was conducted with the framework of science project: "Physical activity for the whole life". The aim of the project are multidirectional activities addressed to Polish-Slovakian students to create a system of taking care about health based on health education among students, selected modifying cardiovascular risk factors monitoring, as well as creating Internet portal to serve those goals. Project was co-fund by European Union from European Regional Development Fund - ERDF, as well as from the government budget by Euro Region Carpathians with the framework of Cross-border Co-operation Programme Republic of Poland - Slovakia Republic 2007-2013. Analysis considered 4584 group of students from University of Rzeszow and University of Presov, Technical University in Rzeszow and State Higher Vocational School in Krosno. The study was conducted from November 2009 to June 2010. The participation in the study was voluntary. The study was conducted using a diagnostics survey method with questionnaire. Chi-square test was used for statistical analysis. Based on results it was claimed that the majority of the studied group of students have never smoked and do not smoke cigarettes. Place of residence was a factor influencing the fact of tobacco smoking. Slovakian students were characterized by more frequent tobacco smoking. Far more often smoke man, both in Polish and Slovakian group. Among Polish students there was a relation between subjective health state assessment and tobacco smoking. The higher self-evaluation, the smaller tobacco smoking prevalence.