Development of a system to sensitively and specifically visualize c-fos mRNA in living cells using bispyrene-modified RNA probes

Reiko Waki; Asako Yamayoshi; Akio Kobori; Akira Murakami

doi:10.1039/c0cc04639f

Development of a system to sensitively and specifically visualize c-fos mRNA in living cells using bispyrene-modified RNA probes

Chem Commun (Camb). 2011 Apr 14;47(14):4204-6. doi: 10.1039/c0cc04639f. Epub 2011 Mar 1.

Authors

Reiko Waki¹, Asako Yamayoshi, Akio Kobori, Akira Murakami

Affiliation

¹ Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.

PMID: 21359378
DOI: 10.1039/c0cc04639f

Abstract

In situ visualization of c-fos mRNA was shown both in fixed cells and in living cells using bispyrene-modified 2'-O-methyl RNA probes (OMUpy2) or phosphorothioate modified OMUpy2 (OMUpy2-S), which was RNA-specific with high sensitivity, and a system for time-lapse imaging of c-fos mRNA was successfully developed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Fluorescent Dyes / chemistry
Humans
Oligoribonucleotides / chemistry
Proto-Oncogene Proteins c-fos / genetics*
Proto-Oncogene Proteins c-fos / metabolism
Pyrenes / chemistry*
RNA Probes / chemistry*
RNA, Messenger / analysis*
RNA, Messenger / metabolism
Time-Lapse Imaging / methods

Substances

Fluorescent Dyes
Oligoribonucleotides
Proto-Oncogene Proteins c-fos
Pyrenes
RNA Probes
RNA, Messenger