Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region

Chem Commun (Camb). 2011 Apr 14;47(14):4162-4. doi: 10.1039/c1cc00078k. Epub 2011 Feb 28.

Abstract

We present a design strategy for fluorescence probes with a high off/on activation ratio in the red wavelength region, based on a novel fluorescein analogue in which the O atom at the 10 position of the xanthene chromophore is replaced with a Si atom. To demonstrate the usefulness of this strategy, we designed and synthesized a red-fluorescent probe for β-galactosidase, and showed that it works in live HEK293 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluoresceins / chemistry*
  • Fluorescent Dyes / chemistry*
  • HEK293 Cells
  • Humans
  • Silicon / chemistry
  • Spectrometry, Fluorescence
  • Xanthenes / chemistry
  • beta-Galactosidase / chemistry
  • beta-Galactosidase / metabolism

Substances

  • Fluoresceins
  • Fluorescent Dyes
  • Xanthenes
  • beta-Galactosidase
  • Silicon