A reporter for dsRNA response in Neurospora crassa

Abstract

In the filamentous fungus Neurospora, the production of dsRNA can elicit a dsRNA-induced transcriptional response similar to the interferon response in vertebrates. However, how fungi sense the expression of dsRNA and activate gene expression is unknown. In this study, we established a dsRNA response reporter system in Neurospora crassa. Using the dsRNA-activated RNA-dependent RNA polymerase gene rrp-3 promoter, we created an expression construct (pRRP-3::Myc-Al-1) and introduced it into al-1(KO) mutant. The test dsRNA efficiently induced pRRP-3::Myc-Al-1 expression in the al-1(KO) mutant, resulting in conidia color switching from white to yellow. These results confirm that the dsRNA response is regulated at the transcriptional level and this reporter system can be used for future studies in dsRNA response in filamentous fungi.