Rapid, simple, and low-cost identification of Candida species using high-resolution melting analysis

Silvia Arancia; Silvia Sandini; Flavia De Bernardis; Daniela Fortini

doi:10.1016/j.diagmicrobio.2010.10.003

Rapid, simple, and low-cost identification of Candida species using high-resolution melting analysis

Diagn Microbiol Infect Dis. 2011 Mar;69(3):283-5. doi: 10.1016/j.diagmicrobio.2010.10.003.

Authors

Silvia Arancia¹, Silvia Sandini, Flavia De Bernardis, Daniela Fortini

Affiliation

¹ Mycoses Unit, Department of Infectious, Parasitic and Immune-mediated Disease, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy. silvia.arancia@iss.it

PMID: 21353953
DOI: 10.1016/j.diagmicrobio.2010.10.003

Abstract

The feasibility of using high-resolution melting analysis (HRMA) was examined for its rapid and simple detection of 5 clinically relevant Candida species (C. albicans, C. glabrata, C. kefyr, C. parapsilosis, and C. guilliermondii). HRMA was able to differentiate clinical Candida species and resulted in being sensitive, reproducible, and inexpensive.

Publication types

Evaluation Study

MeSH terms

Candida / classification*
Candida / genetics*
Candidiasis / diagnosis
Candidiasis / microbiology
DNA, Fungal / chemistry*
Humans
Mycological Typing Techniques / economics
Mycological Typing Techniques / methods*
Nucleic Acid Denaturation*
Polymerase Chain Reaction
Reproducibility of Results
Sensitivity and Specificity
Sequence Analysis, DNA / methods

Substances

DNA, Fungal