3-Diethylaminopropyl-bearing glycol chitosan as a protein drug carrier

Colloids Surf B Biointerfaces. 2011 Jun 1;84(2):585-90. doi: 10.1016/j.colsurfb.2011.01.017. Epub 2011 Jan 21.

Abstract

Polysaccharidic nanogels were fabricated with bovine serum albumin (BSA) and a glycol chitosan (GCS) grafted with functional 3-diethylaminopropyl (DEAP) groups. These nanogels were investigated to evaluate their cellular uptake in HeLa cells and in vivo fate in nude mice tumor model. Unlike free BSA, GCS-g-DEAP/BSA nanogels improved cellular uptake of BSA. Furthermore, this system led to an enhanced blood circulation and a high accumulation of BSA in the tumor site. Our collective results strongly support that GCS-g-DEAP/BSA nanogel is a potential carrier system for high molecular weight proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Propanol / chemistry*
  • Amines / chemistry*
  • Animals
  • Cattle
  • Cell Line, Tumor
  • Chitosan / chemistry*
  • Drug Delivery Systems* / methods
  • Female
  • Flow Cytometry
  • Humans
  • Mice
  • Mice, Nude
  • Models, Molecular
  • Molecular Structure
  • Nanogels
  • Neoplasms / drug therapy
  • Polyethylene Glycols / chemistry
  • Polyethyleneimine / chemistry
  • Serum Albumin, Bovine / chemistry*

Substances

  • Amines
  • Nanogels
  • glycol-chitosan
  • polyethylene glycol polyethyleneimine nanogel
  • Serum Albumin, Bovine
  • Polyethylene Glycols
  • Polyethyleneimine
  • Chitosan
  • 1-Propanol