Fast preparation of fungal DNA for PCR screening

Kuang-Hung Liu; Yu-Ling Yeh; Wei-Chiang Shen

doi:10.1016/j.mimet.2011.01.029

Fast preparation of fungal DNA for PCR screening

J Microbiol Methods. 2011 May;85(2):170-2. doi: 10.1016/j.mimet.2011.01.029. Epub 2011 Feb 17.

Authors

Kuang-Hung Liu¹, Yu-Ling Yeh, Wei-Chiang Shen

Affiliation

¹ Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan.

PMID: 21315113
DOI: 10.1016/j.mimet.2011.01.029

Abstract

Rapid DNA preparation for the quick screening is highly demanded in diverse research fields. Here, we combined an extraction buffer and heat treatment to generate DNA templates from yeast and filamentous fungal materials for PCR. This method may be widely applicable to diverse fungal species in clinical and basic studies.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Chemical Fractionation / methods*
DNA Primers / genetics
DNA, Fungal / genetics
DNA, Fungal / isolation & purification*
Fungi / chemistry
Fungi / genetics*
Fungi / isolation & purification
Humans
Mycoses / microbiology
Polymerase Chain Reaction

Substances

DNA Primers
DNA, Fungal