Multiplex cancer cell detection by SERS nanotags with cyanine and triphenylmethine Raman reporters

Chem Commun (Camb). 2011 Mar 28;47(12):3514-6. doi: 10.1039/c0cc05265e. Epub 2011 Feb 9.

Abstract

SERS nanotags have been prepared to accomplish the multiplex detection of cancer cells. Herein we evaluated the adequacy of lipoic acid-containing cyanine derivatives (Cy3LA and Cy5LA) to function as multiplex partners with a triphenylmethine Raman reporter (B2LA) under a single excitation wavelength. SERS experiments enabled the multiplex recognition of two different cancer cells with antibody-conjugated nanotags that were derivatized with optimized cyanine and triphenylmethine reporters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / immunology
  • Carbocyanines / chemistry*
  • Carbocyanines / metabolism
  • Cell Line, Tumor
  • Cell Separation / methods*
  • Coloring Agents / chemistry*
  • Coloring Agents / metabolism
  • Humans
  • Nanostructures*
  • Peptides / chemistry*
  • Peptides / metabolism
  • Spectrum Analysis, Raman / methods*
  • Surface Properties

Substances

  • Antibodies
  • Carbocyanines
  • Coloring Agents
  • Peptides
  • methinin